Use this url to cite publication: https://hdl.handle.net/20.500.12512/99445
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The Effect of cranberry and chokeberry pomace extracts on cancer cell viability, clonogenic activity and cell-to-cell coupling / Kęstutis Gabrielius Šaulys, Gintarė Jančiukė, Ieva Sarapinienė
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
The Effect of cranberry and chokeberry pomace extracts on cancer cell viability, clonogenic activity and cell-to-cell coupling / Kęstutis Gabrielius Šaulys, Gintarė Jančiukė, Ieva Sarapinienė
Publisher (trusted)
Lietuvos fiziologų draugija |
Date Issued
Date Issued |
---|
2019-12-09 |
Extent
p. 20-20.
Is part of
3rd Meeting of the Baltic Physiological Societies : December 9–10, 2019 Kaunas : abstracts / Lithuanian, Latvian and Estonian Physiological Societies. Lithuanian University of Health Sciences. [Kaunas] : Lietuvos fiziologų draugija, 2019. ISBN 9789955156369.
Version
Originalus / Original
Description
ISBN 978-9955-15-636-9.
Field of Science
Abstract
The extracts of various berries are rich of flavonoids, phenolic acids, tannins, vitamins, stilbenes and other compounds that are known to possess anticancer activities. The aim of this study was to evaluate the composition of cranberry and chokeberry pomace extracts (CRE and CHE), and to examine their effect on cancer cell viability, colony formation and cell-to-cell coupling. MTT and flow cytometry assays revealed that all tested cell lines (DLD-1, HCT116, HeLa expressing exogenous connexin 45 (HeLa-Cx45) and Novikoff) were more sensitive to CHE (40-1000 μg/ml) treatment compared to CRE (40-1000 μg/ml) in reducing cancer cell viability and proliferation. Clonogenic assay showed that CHE also was more effective in suppression of cancer cell colony formation. The strongest effect was achieved using 1000 μg/ml of CHE. Impaired intercellular communication of cancer cells is related with their oncogenic properties. We measured gap junction (GJ) conductance (gj) between HeLa cells expressing exogenous connexin 45, and Novikoff cells expressing endogenous connexin 43 by dual whole-cell patch-clamp technique. CRE (100 μg/ml) stimulated gj of Cx45 GJ channels but had no effect on Cx43 channel gj. In contrast, CHR (200 μg/ml) had no effect on Cx45 GJ channels but decreased Cx43 channel gj. Taken together our results suggest that CRE and CHE, both possess anticancer activity but differently regulate cell-to-cell coupling through Cx45 and Cx43 GJ channels.
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9789955156369
Other Identifier(s)
(LSMU ALMA)990001000490107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)