Use this url to cite publication: https://hdl.handle.net/20.500.12512/99260
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Comparison of in vivo and in vitro response in identical parasitic infection in goats and sheep / M. Komáromyová, M. Babják, A. Königová, M. Urda Dolinská, Š. Megyesi, T. Kupčinskas, G. von Samson-Himmelstjerna, M. Várady
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
Komáromyová, Michaela | Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia |
Babjak, Michal | Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia |
Königová, Alžbeta | Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia |
Urda Dolinská, Michaela | Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia |
Megyesi, Štefánia | Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia |
Samson-Himmelstjerna, Georg von | Freie Universität Berlin, Institute for Parasitology and Tropical Veterinary Medicine, Berlin, Germany |
Várady, Marián | Institute of Parasitology, Slovak Academy of Sciences, Košice, Slovakia |
Title
Comparison of in vivo and in vitro response in identical parasitic infection in goats and sheep / M. Komáromyová, M. Babják, A. Königová, M. Urda Dolinská, Š. Megyesi, T. Kupčinskas, G. von Samson-Himmelstjerna, M. Várady
Date Issued
Date Issued |
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2019-08-27 |
Extent
p. 47-47.
Is part of
Joint COMBAR – ACSRPC (COMBatting Anthelmintic Resistance in Ruminants – American Consortium for Small Ruminant Parasite Control) meeting „Anthelmintic resistance in ruminants: Who cares?“ : August 27-29th, 2019, Ghent, Belgium : abstract book / Ghent University. .
Version
Originalus / Original
Description
Poster presentations: working group 1.
Field of Science
Abstract
The objective of the study was to compare in vivo faecal egg count reduction test (FECRT), in vitro Egg Hatch Test (EHT) and molecular determination of codon 200 benzimidazole-resistance associated allele frequencies in H. contortus obtained from infected goats and sheep. In this study 54 lambs and 54 goat kids were each infected with mixture of susceptible and benzimidazole-resistant H. contortus isolates. Composite infection doses consisted of 10, 20, 30, 40, 60 and 80 % of the respective resistant isolate. The results of the in vivo FECRT showed that albendazole treatment caused infection reduction which correlated with eggs per gram values in goats. The results of ED50 values in the in vitro EHT varied from 0.12±0.01 to 28.05±20.87 μg/ml TBZ in sheep and from 0.09±0.01 to 15.63±12.10 μg/ml TBZ in goats. The results of PyrosequencingTM revealed that the percentages of the susceptible (TTC) and resistant (TAC) alleles in H. contortus L3 larvae derived from different groups of sheep and goats were closely related with the respective composition of larvae which have been used for infection. The results of the in vivo and in vitro tests in both species confirmed solid base for an estimation of clinical resistance in vivo. Surprisingly, comparable results obtained after treatment with the same dose rate indicate a similar bioavailability of the drug in sheep and goats.
Type of document
type::text::conference output::conference proceedings::conference paper
Other Identifier(s)
(LSMU ALMA)990000994430107106
Coverage Spatial
Belgija / Belgium (BE)
Language
Anglų / English (en)
Funding(s)
Funding(s) | Grant No |
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Slovak Research and Development Agency | Project APVV-14-0169 |