Use this url to cite publication: https://hdl.handle.net/20.500.12512/97684
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The Impact of C1q on Wnt pathway and PPAR-δ activation related to breast cancer cell viability / Valeryia Mikalayeva, Ieva Sarapinienė, V. Arvydas Skeberdis, Sergio Bordel
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Title
The Impact of C1q on Wnt pathway and PPAR-δ activation related to breast cancer cell viability / Valeryia Mikalayeva, Ieva Sarapinienė, V. Arvydas Skeberdis, Sergio Bordel
Publisher (trusted)
Lithuanian Biochemical Society |
Date Issued
Date Issued |
---|
2018-06-26 |
Extent
p. 32-32, no. 15.
Is part of
XVth International Conference of the Lithuanian Biochemical Society : The programme and abstract book : Dubingiai, June 26-29, 2018 / Lithuanian Biochemical Society. Kaunas : Lithuanian Biochemical Society, 2018. ISBN 9786099603001.
Version
Originalus / Original
Series/Report no.
Poster presentations (session I).
Description
ISBN 978-609-96030-0-1.
Field of Science
Abstract
It has been recently shown that plasma concentration of C1q protein increases with age and is capable of triggering the Wnt signaling pathway that in turn is known to play an important role in cancer development. Transcription factor PPAR-δ mediates the increased lipid oxidation and higher capability of cancer cells to avoid the apoptosis. In this work we experimentally examined the hypothesis that C1q induce the Wnt-dependent up-regulation of PPAR-δ. The ability of activated Wnt pathway to evoke resistance to apoptosis induced by loss of attachment was evaluated. PolyHEMA coated cell culture dishes were used for low attachment conditions. Cell viability was measured using Annexin V/ 7-AAD reagent kit and mitochondrial Δψm was measured using JC1 dye by flow cytometry. Our results showed that activation of Wnt pathway with C1q increased β-catenin and PPAR-δ protein levels in breast cancer cells. Lower rate of apoptosis was determined in C1q treated cells under low-attachment conditions. C1q treatment as well as low-attachments conditions altered mitochondrial Δψm.
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9786099603001
Other Identifier(s)
(LSMU ALMA)990000961890107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)