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Accumulation of long chain fatty acids decreases the cardioprotective effect of KATP channel openers / D. M. Kopustinskiene, Z. Polianskyte, A. Toleikis
Type of publication
Tezės Web of Science duomenų bazėje / Theses in Web of Science database (T1a1)
Title
Accumulation of long chain fatty acids decreases the cardioprotective effect of KATP channel openers / D. M. Kopustinskiene, Z. Polianskyte, A. Toleikis
Publisher (trusted)
Is Referenced by
Date Issued
Date Issued |
---|
2005-07-02 |
Extent
p. 57-57, no. N5-013P.
Is part of
The FEBS journal : 30th FEBS congress and 9th IUBMB conference The protein World : abstracts : Budapest, Hungary, 2-7 July 2005. Oxford, UK : Published by Blackwell Pub. on behalf of the Federation of European Biochemical Societies, 2005, vol. 272, suppl. 1, July.
Version
Originalus / Original
Series/Report no.
Abstracts
Description
eISSN 1742-464X.
Field of Science
Abstract
The oxidation of fatty acids serves as the main energy source for cardiomyocytes. However, in ischemic tissue fatty acid oxidation is inhibited, resulting in accumulation of long chain fatty acids and their CoA derivatives. In this study, we investigated the influence of fatty acids on the effects of KATP channel openers on isolated rat heart mitochondria. Mitochondrial respiration rates were recorded by the means of Clark-type oxygen electrode in the isotonic KCl medium (37 °C), using pyruvate and malate (6–6 mm) or fatty acids - palmitoyl-L-carnitine (9 mkm) and malate (240 mkm) or palmitoyl-CoA (5 mkm; + L-carnitine (2 mm)) and malate (240 mkm) as substrates. The results showed that KATP channel openers diazoxide (300 mkm) and pinacidil (300 mkm) similarly increased (by ~100%) the state 2 respiration rate of mitochondria, oxidizing either pyruvate and malate or fatty acids. In contrast, when palmitoyl-CoA (2.5 mkm) was not oxidized (medium devoid of carnitine), it suppressed the uncoupling effect of KATP channel openers in mitochondria, respiring on pyruvate and malate. Thus, during ischemia, the effects of KATP channel openers could decrease due to accumulation of physiological inhibitor of adenine nucleotide translocase - palmitoyl-CoA. Since uncoupling is proposed as one of the mechanisms of pharmacological cardioprotection, our results suggest that higher concentrations of KATP channel openers might be required to protect cardiomyocytes from ischemic injuries.
Type of document
type::text::conference output::conference proceedings::conference paper
ISSN (of the container)
1474-3833
Other Identifier(s)
(LSMU ALMA)990000647150107106
Coverage Spatial
Vengrija / Hungary (HU)
Language
Anglų / English (en)