Use this url to cite publication: https://hdl.handle.net/20.500.12512/20284
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Factors influencing the reliability and preciseness of trace elements ICP-MS analysis in biological material / Dalė Baranauskienė, Vaida Šimakauskienė, Donata Drūlytė, Monika Tirlikaitė, Inesa Ambraškaitė, Rima Naginienė
Type of publication
Konferencijų tezės nerecenzuojamame leidinyje / Conference theses in non-peer-reviewed publication (T2)
Author(s)
Title
Factors influencing the reliability and preciseness of trace elements ICP-MS analysis in biological material / Dalė Baranauskienė, Vaida Šimakauskienė, Donata Drūlytė, Monika Tirlikaitė, Inesa Ambraškaitė, Rima Naginienė
Publisher (trusted)
Lithuanian Biochemical Society |
Date Issued
Date Issued |
---|
2018-06-26 |
Extent
p. 25-25, no. 4.
Is part of
XVth International Conference of the Lithuanian Biochemical Society : The programme and abstract book : Dubingiai, June 26-29, 2018 / Lithuanian Biochemical Society. Kaunas : Lithuanian Biochemical Society, 2018. ISBN 9786099603001.
Version
Originalus / Original
Series/Report no.
Poster presentations (session I).
Description
ISBN 978-609-96030-0-1.
Field of Science
Keywords
Abstract
Analysis of trace elements in biological material is a challenging task due to very small concentrations present in the matrix. Inductively coupled plasma mass spectrometry (ICP-MS) is a most suitable technique for the purpose because it exhibits a good precision, excellent sensitivity, and multielemental capability, so that majority of trace elements can be reliably analysed simultaneously. Therefore, the technique of material preparation and effect of storage duration before the analysis also have crucial impact on the reliability and preciseness of trace elements analysis. The aim of the study was to explore different biological material pretreatment techniques, storage duration effect before the analysis and to find the most reliable ICP-MS analysis mode that exhibits the most accurate reading of lead, cadmium, selenium and manganese in blood and urine. The research was conducted using the NexION 300D ICP-MS (PerkinElmer, USA) equipped with nickel cones and quartz cyclonic spray chamber as a sample introduction system and applying three different modes: Standard mode, Kinetic energy discrimination (KED) mode and Dynamic reaction cell (DRC) mode, and following manufacturer recommendations. Blood and urine samples were pretreated by direct dilution or mineralization (Microwave 3000, Anton Paar, Austria). The analyses of trace elements were performed in certified reference materials: Seronorm™ Trace Elements Urine Blank, Seronorm™ Trace Elements Whole Blood L-II (SERO AS, Norway), Clin Check® Trace Elements Whole Blood L-II (RECIPE, Germany). Each sample was analysed three times investigating the reliability and preciseness, analyses were repeated after 1 and 5 months. The combined results of blood and urine pretreatment technique, ICP-MS analysis using different analysis mode of selected trace elements – lead, cadmium, selenium, manganese, and storage duration effect will be presented in details in the poster. We conclude that all – the pretreatme
Type of document
type::text::conference output::conference proceedings::conference paper
ISBN (of the container)
9786099603001
Other Identifier(s)
(LSMU ALMA)990000961860107106
Coverage Spatial
Lietuva / Lithuania (LT)
Language
Anglų / English (en)