Use this url to cite publication: https://hdl.handle.net/20.500.12512/110050
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Detection of genes responsible for biofilm formation in blood of patients with ischaemic coronary artery disease / Kaminskaite Kornelija, Tatarunas Vacis, Giedraitiene Agne
Type of publication
Tezės kitame recenzuojamame leidinyje / Theses in other peer-reviewed publication (T1e)
Title
Detection of genes responsible for biofilm formation in blood of patients with ischaemic coronary artery disease / Kaminskaite Kornelija, Tatarunas Vacis, Giedraitiene Agne
Publisher (trusted)
Lithuanian University of Health Sciences |
Multidisciplinary Digital Publishing Institute (MDPI AG) |
Date Issued
Date Issued |
---|
2021-04-23 |
Extent
p. 259-259.
Is part of
Medicina : Abstracts accepted for the International Scientific Conference on Medicine organized within the frame of the 79th International Scientific Conference of the University of Latvia : [23-24 April], Riga, Latvia / Editor-in-Chief Edgaras Stankevičius. Kaunas ; Basel : LSMU ; MDPI, 2021, vol. 57, suppl. 1.
Version
Originalus / Original
Description
Poster presentations. Basic Medical Science
Buvo eISSN 1648-9233.
Field of Science
Abstract
Background. It is known that bacteria are involved in the development and progression of atherosclerosis by forming biofilms within arterial plaques. It may lead to a rupture of unstable plaque and myocardial ischaemia. Clinically important microorganisms forming biofilms on or inside the human body secrete bacterial DNA. It has been shown that wcaF gene encoded for colanic acid (a key polysaccharide in E. coli biofilm extracellular polymeric substance) is up-regulated in mature biofilms. Gene papC is essential for the formation of P fimbriae for adherence to uroepithelial cells when a gene sdhC, involved in the production of fumarate, has been reported to stimulate biofilm formation. A modern real-time PCR may allow to determine these genes rapidly. Aim. The aim of the current study was to detect bacterial genes, responsible for a formation of a biofilm, in whole blood of patients with ischaemic coronary artery disease. Methods. Altogether 80 blood samples of patients (40 women and 40 men) with cardiovascular diseases were tested for bacterial genes wcaF, sdhC and papC. Identification of a biofilm encoding genes was performed by using real-time PCR. Reference strain of E. coli ATCC 52922 was used as a positive control. Results. Bacterial genes were detected in nearly 20% of whole blood samples (gene wcaF in 12.5%, gene sdhC in 7.5% of all samples analysed when papC gene was not detected). There were determined differences in genes prevalence (due to a low sample size differences were not significant) according to gender. Conclusion. The data obtained suggest that bacterial genes responsible for a biofilm formation might be detected in patients with ischaemic coronary artery disease, more often in men than women [...].
Type of document
type::text::conference output::conference proceedings::conference paper
ISSN (of the container)
1010-660X
1648-9144
Other Identifier(s)
(LSMU ALMA)990001033950107106
Coverage Spatial
Latvija / Latvia (LV)
Language
Anglų / English (en)
Affiliation(s)
Journal | IF | AIF | AIF (min) | AIF (max) | Cat | AV | Year | Quartile |
---|---|---|---|---|---|---|---|---|
Medicina-Lithuania | 2.948 | 8.28 | 8.28 | 8.28 | 1 | 0.356 | 2021 | Q3 |
Journal | IF | AIF | AIF (min) | AIF (max) | Cat | AV | Year | Quartile |
---|---|---|---|---|---|---|---|---|
Medicina-Lithuania | 2.948 | 8.28 | 8.28 | 8.28 | 1 | 0.356 | 2021 | Q3 |
Journal | Cite Score | SNIP | SJR | Year | Quartile |
---|---|---|---|---|---|
Medicina (Lithuania) | 2.7 | 0.959 | 0.536 | 2021 | Q2 |