Laboratory of Biochemistry
|Address:Laboratory of BiochemistryNeuroscience InstituteLithuanian University of Health SciencesEiveniu st. 4Kaunas LT-50009Lithuania
Phone: +370 37 302968
Head of the laboratory:
prof. Vilmantė Borutaitė, PhD, email@example.com
prof. Ramunė Morkūnienė, PhD, firstname.lastname@example.org
prof. Sonata Trumbeckaitė, PhD, email@example.com
prof. Rasa Banienė, PhD, firstname.lastname@example.org
Julius Liobikas, PhD, email@example.com
Daiva Majienė, PhD, firstname.lastname@example.org
Aistė Jekabsone, PhD, email@example.com
Odeta Arandarčikaitė, PhD, firstname.lastname@example.org
Kristina Škėmienė, PhD, email@example.com
Paulius Čižas, PhD, firstname.lastname@example.org
Silvija Jankevičiūtė, PhD student, email@example.com
Title: The investigation of cell death and protective mechanisms in models of brain ischemia, neurodegenerative and other diseases
Protein aggregates containing beta amyloid (Aβ) and other multimeric proteins are critical factors in the pathogenesis of neurodegenerative diseases (including Alzheimer's disease) by causing synaptic dysfunction, neuronal death and loss. Molecular mechanism by which protein aggregates cause death of brain cells and activation of neuroimmune processes will be investigated. Alongside, search and investigation of possible pharmacological modulators of the processes involved will be performed.
The study also will focus on mechanisms of ischemic mitochondrial damage in brain tissue. The possible sites of ischemic damage in mitochondria will be determined and the possible protective farmacological agents against ischemic mitochondrial injury will be tested.
We are going to determine which compounds (synthetic and natural), acting separately or in combination with pahrmacological compounds, are able to suppress viability of brain cancer cells, mitosis/interphase cycle, proliferation and migration of cells in the model systems. We are planning to reveal possible molecular mechanisms of action of those compounds in model systems. We expect that obtained data will pave the way for the in vivo studies on mice glioma allograft and human/mice xenograft models, and will allow us to confirm and specify in vitro data.
The Laboratory has expertise in primary neuronal and transformed cell cultures; cell biology; potentiometric methods using oxygen, nitric oxide and ion-selective electrodes; spectroscopy, fluorescent microscopy, protein chemistry, DIGE, etc.
2016-2020 - COST action MITOEAGLE “Mitochondrial mapping: Evolution - Age - Gender - Lifestyle - Environment"
2015-2018 – collaboration in the project No SEN-01/2015 „Optimizing the effectiveness of Hyperthermic chemotherapy by modulation of heat shock proteins, energy resources and adhesion in malignant cells“
2015-2016 – collaboration in EU Horizon 2020 project No 664339 HEALTH-TECH “Centre of Excellence in Science and Technology for Healthy Ageing"
2015 – project "In vitro investigation of interaction of cells with customer's prototypic hydrogel samples", managed by Lithuanian Business Promotion Agency
2013-2015 – COST action FA 1005 INFOGEST "Improving health properties of food by sharing our knowledge on the digestive process"
2012-2015 – contribute in the Global grant project* VP1-3.1-ŠMM-07-K-02-062 "Energetic, genetic and epigenetic mechanisms of resistance to chemotherapy induced apoptosis in pancreatic cancer", (leaded by dr. Ž.Dambrauskas)
2011-2015 – Global grant project* VP1-3.1-ŠMM-07-K-01-130 “Mitochondria, nitric oxide and cytochrome c reducing agents in protection against brain and heart ischemia-reperfusion injury” (leaded by prof. V.Borutaitė)
2012-2014 – project LIG-04/2012 MALPAMA "Molecular mechanisms of pathogenesis of Alzheimer’s disease” (n collaboration with Institute of Biochemistry, and Institute of Biotechnology of Vilnius University, Department of Neurology of Lithuanian University of Health Sciences Hospital), funded by Research Council of Lithuania
2012-2014 – contribute in the project LIG-12002 STELLATA „Investigation of activity and redox status of Pancreatic Stellate Cells", funded by the Research Council of Lithuania
The Laboratory participates in a project of the Advanced Pharmaceutical and Health Technologies Centre of Lithuanian University of Health Sciences. The researchers have access and are actively using open access facilities of the Laboratory for Pharmacological Regulation of Pathological Processes and Research of Drug Metabolism.
Dr. Guy Brown, Department of Biochemistry, University of Cambridge, UK
Dr. Erich Gnaiger, Department of General, Visceral and Transplant Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, Innsbruck, Austria
Other important information
In 2008, prof. A.Toleikis, prof. V.Borutaite, prof. V.Mildaziene (former member of the laboratory) were awarded Lithuanian National Price for Science.
Members of the Laboratory in collaboration organized the 4th MiPsummer School on Mitochondrial Physiology in Druskininkai, Lithuania on June 10-16, 2010.
|2011 - 2015 main publications (149KB)|
Updated on 19th september 2016