Spausdinti

Laboratory of Membrane Biophysics

 

Laboratory of Membrane Biophysics

 Jonas Jurevičius,

Head of laboratory, Head researcher, tel. 302877,

e-mail: jonas.jurevicius@lsmuni.lt.

Personnel:

 Regina Mačianskienė dr., Head researcher

 Irma Martišienė dr. , Researcher

 Rimantas Treinys dr.,Senior researcher

 Mantė Almanaitytė, Junior researcher

 Inga Andriulė, Junior researcher

 Antanas Navalinskas,  Senior engineer

 Vanda Burbulytė,  Engineer

 Kęstutis Runkevičius,  Engineer

 Virginija Luckienė, preparator

 

The main research activities of the laboratory

  • Remodeling of ion channels and intracellular communication in the heart
  • Novel mechanisms of arrhythmias – from the cell to the whole heart
  • Development of new imaging modalities for optical mapping in vivo and obtaining proof-of-concept of novel therapeutic approaches.

 We investigate what’s changes in electrophysiological properties of ion channels (Na+, L-type Ca2+, various K+, and novel TRPM7, etc.) in the heart with ischemia, arrhythmias, heart failure, etc.: current density, kinetics, sensitivity to pharmacological regulation. Using biophysical, molecular and pharmacological approaches we elucidate ion channel function under physiological and pathological conditions and its relationship to cell signaling.

We develop new imaging modalities for evaluation of optical/electrical signal in vivo, and evaluate evolution of possible mechanisms of arrhythmias, induced by ischemia, in particular, changes of electrical signal in different cell layers of myocardium (on Langendorf-perfused heart from animal model): sensitivity to ischemia, changes in both ion channels and membrane potential, in interaction between ischemia and remodeling of ion channels, in intracellular communication, in electrical wave propagation upon ischemia in various cell layers, in sensitivity to pharmacological drugs. We evaluate what of them are more pronounced, and how the origin of arrhythmias could be affected; this could change strategy for treatment of various ventricular arrhythmias.

In cooperation with dr. Rodolphe Fischmeister (The Faculty of Pharmacy, at 11th University of Paris, France), we study cellular and molecular mechanisms of calcium signaling in ischemic myocardium and β-adrenergic regulation of L-type calcium current of experimental animals and human heart.

In cooperation with dr. Arkady M. Pertsov (SUNY Upstate Medical University, Department of Pharmacology, Syracuse, NY, USA), we gather the scientific and technological approaches. We developed of in situ optical mapping model (mapping of “non-explanted heart”) in small (rabbits) and big (pigs) animals as well as the problems of the usage of NIR probes in blood-perfused myocardium. Such experimental design is completely new and optical mapping experiments of the heart in situ have not been performed. We study mechanisms and dynamics of T-wave alternans in ischemic heart models (Langendorff-perfused and in situ (intact) hearts during induced ischemia).

In cooperation with dr. Kanigula Mubagwa (The Katholic University of Leuven, Belgium) we investigate cellular and molecular mechanisms of the TRPM7 cation channels and of intracellular magnesium in the failing myocardium.

Technical facilities of the laboratory enable us to perform collaborative research projects with other laboratories working in different scientific fields. In the past, we have carried preclinical drug examination by testing new synthesized compounds for companies such as Institute of Organic Synthesis (Latvia) and Karo-Bio (Sweden). Together with colleagues from 11thUniversity of Paris (France), SUNY Upstate Medical University (Syracuse, NY, USA) and KUL (Belgium) we perform electrophysiology studies of intracellular signaling of cardiac cell/heart viability, arrhythmogenesis and other properties under stress conditions. These allow developing new bio-markers, new technical strategies as well as new targets of pharmacological drugs that would be acceptable for diseased heart.

 Projects:

  1. National Research Programme “Healthy ageing” project “Age-dependent lipid metabolism and cancer cell viability” supported by Lithuanian Research Council grant (SEN-15/2015); 2015-2018; 200.000 Eur.; Principal investigator – Prof. dr. Rimantas Benetis.
  2. Researcher teams’ project “Mechanisms of calcium cycling in ischaemic myocardium” supported by Lithuanian Research Council grant (MIP-058/2015); 2015-2017; 95.277 Eur.; Principal investigator – Habil. dr. Jonas Jurevičius.
  3. Global grant project “Investigation of T-wave alternants formation mechanisms during ischemia in explanted and in situ heart models” (No. VP1-3.1- ŠMM-07-K-01-121); 2011-2015; 1 597 847 Lt.; Principal investigator – prof. Arkady M. Pertsov, Co-principal investigator – Habil. dr. Jonas Jurevičius.
  4. National Complex Programme “Molecular mechanisms of the ischemic heart disease” supported by European Social Fund (Project Code Number VP1-3.1-ŠMM-0.8-K-01-022), 2012-2014, 746.623 Lt.; Principal investigator – dr. Regina Mačianskienė.

 

International collaboration

  • Dr. Rodolphe Fischmeister,  Laboratory of Cellular and Molecular Cardiology of Faculty of Pharmacy of 11th University of Paris, France.
  • Dr. Kanigula Mubagwa and, Dr. Karin Sipido,Division of Experimental Cardiac Surgery and Division of Experimental Cardiology, Department of Cardiovascular Diseases, University of Leuven, Leuven, Belgium.
  • Dr. John Starkus. Pacific Biomedical Research Centre of the University of Hawaii, Honolulu, Hawaii, USA.
  • Dr. Arkady Pertsov. SUNY Upstate Medical University, Department of Pharmacology; Laboratory of Bioengineering, Syracuse, NY, USA.
  • Dr.Lothar A. Blatter & Dr. Giedrius Kanaporis. Department of Physiology & Biophysics, Rush University Medical Center, Chicago, USA.

 

Other important information

In 2009, habil.dr.. Jonas Jurevičius and dr. Arvydas V. Skeberdis  (former member of the laboratory) were awarded with Lithuanian National Price for Science.

  

Main publications (2017-2013):

2017 -2016 

  1. Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart / Regina Mačianskienė, Mantė Almanaitytė, Rimantas Treinys, Antanas Navalinskas, Rimantas Benetis, Jonas Jurevičius // Scientific reports. London: Nature Publishing Group. ISSN 2045-2322. 2017, vol. 7, no. 1, p. 1-12. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; 1. Internet access: 
  2. Loss-of-activity-mutation in the cardiac chloride-bicarbonate exchanger AE3 causes short QT syndrome / Kasper Thorsen, Vibeke S. Dam, Kasper Kjaer-Sorensen, Lisbeth N. Pedersen, V. Arvydas Skeberdis, Jonas Jurevičius, Rimantas Treinys, Ida M.B.S. Petersen, Morten S. Nielsen, Claus Oxvig, J. Preben Morth, Vladimir V. Matchkov, Christian Aalkjær, Henning Bundgaard, Henrik K. Jensen // Nature communications. London: Nature Publishing Group. ISSN 2041-1723. 2017, vol. 8, no. 1, p. 1-10. Science Citation Index Expanded (Web of Science); MEDLINE; DOAJ; 0.529.  Internet access.:
  3. Modulation of human cardiac TRPM7 current by extracellular acidic pH depends upon extracellular concentrations of divalent cations / Regina Mačianskienė, Mantė Almanaitytė, Aistė Jekabsone, Kanigula Mubagwa // PLoS One. San Francisco: Public Library of Science. (Research article). ISSN 1932-6203. 2017, vol. 12, no. 1, p. 1-19.Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; AGRICOLA; 0.866. Internet access :
  4. Metabolic inhibition reduces cardiac L-type Ca2+ channel current due to acidification caused by ATP hydrolysis / Giedrius Kanaporis, Rimantas Treinys, Rodolphe Fischmeister, Jonas Jurevičius // PLoS One. San Francisco: Public Library of Science. (Research article). ISSN 1932-6203. 2017, vol. 12, no. 8, p. 1-17.Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; AGRICOLA; 0.866. Internet access :
  5. Differences in the control of basal L-type Ca2+ current by the cyclic AMP signaling cascade in frog, rat, and human cardiac myocytes / Rimantas Treinys, Andrius Bogdelis, Lina Rimkutė, Jonas Jurevičius, Vytenis Arvydas Skeberdis // Journal of physiological sciences : JPS. Tokyo: Springer. (Original paper). ISSN 1880-6546. 2016, vol. 66, no. 4, p. 327-336. Science Citation Index Expanded (Web of Science); MEDLINE; Chemical Abstracts Service (CAS); CAB International; Scopus; 0.8. Internet access:
  6. Voltage-sensitive fluorescence of indocyanine green in the heart / Irma Martišienė, Regina Mačianskienė, Rimantas Treinys, Antanas Navalinskas, Mantė Almanaitytė, Dainius Karčiauskas, Audrius Kučinskas, Ramunė Grigalevičiūtė, Vilma Zigmantaitė, Rimantas Benetis, Jonas Jurevičius // Biophysical journal. Cambridge, MA: Cell Press. ISSN 0006-3495. 2016, vol. 110, no. 3, p. 723-732. Science Citation Index Expanded (Web of Science); MEDLINE; 1.102.  Internet access:

2015 -2013 

  1. Exogenous connexin43-expressing autologous skeletal myoblasts ameliorate mechanical function and electrical activity of the rabbit heart after experimental infarction / Antanavičiūtė I, Ereminienė E, Vysockas V, Račkauskas M, Skipskis V, Rysevaitė K, Treinys R, Benetis R, Jurevičius J, Skeberdis VA // International journal of experimental pathology. Oxford, OX: Blackwell Scientific Publications. (Original article). ISSN 0959-9673. 2015, vol. 96, no. 1, p. 42-53. Science Citation Index Expanded (Web of Science); MEDLINE; Index Veterinarius; CAB Abstracts® (CABI); 1.342. Internet access:
  2. Evolution of action potential alternans in rabbit heart during acute regional ischemia / Martišienė I, Jurevičius J, Vosyliūtė R, Navalinskas A, Treinys R, Mačianskienė R, Benetis R, Matiukas A, Pertsov AM // BioMed research international. New York: Hindawi Publishing Corporation. (Research article). ISSN 2314-6133. 2015, vol. 2015, p. 1-12. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; 1.1.  Internet access:
  3. Evaluation of excitation propagation in the rabbit heart: optical mapping and transmural microelectrode recordings / Regina Mačianskienė, Irma Martišienė, Antanas Navalinskas, Rūta Vosyliūtė, Rimantas Treinys, Birutė Vaidelytė, Rimantas Benetis, Jonas Jurevičius // PLoS One. San Francisco: Public Library of Science. (Research article). ISSN 1932-6203. 2015, vol. 10, no. 4, p. 1-18. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; Chemical Abstracts Service (CAS); AGRICOLA; PyscINFO; Zoological Records; DOAJ; 0.875. Internet access :
  4. The Role of external Ca(2+) in the action of Ca(2+) - channel agonists and antagonists on isolated human thoracic arteries / Garaliene V, Barsys V, Giedraitis S, Benetis R, Krauze A // Journal of physiology and pharmacology. Krakow: Polish Physiological Society. (Original article). ISSN 0867-5910. 2014, vol. 65, no. 1, p. 25-31. Science Citation Index Expanded (Web of Science); MEDLINE; 0.4. Internet access :
  5. β3-adrenergic regulation of L-Type Ca2+ current and force of contraction in human ventricle / Treinys R, Zablockaitė D, Gendvilienė V, Jurevičius J, Skeberdis VA // Journal of membrane biology. New York: Springer. ISSN 0022-2631. 2014, vol. 247, iss. 4, p. 309-318. Science Citation Index Expanded (Web of Science); MEDLINE; 0.6.  Internet access:
  6. DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration / Kaselis A, Treinys R, Vosyliūtė R, Satkauskas S // Cellular and molecular neurobiology. New York: Kluwer Academic/Plenum Publishers. (Original research). ISSN 0272-4340. 2014, vol. 34, no. 2, p. 289-296. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; AGRICOLA; CAB Abstracts; 0.354.  Internet access:
  7.  R-Type calcium channels are crucial for semaphorin 3A-induced DRG axon growth cone collapse / Treinys R, Kaselis A, Jover E, Bagnard D, Satkauskas S // PLoS One. San Francisco: Public Library of Science. ISSN 1932-6203. 2014, vol. 9, no. 7, p. 1-14. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; AGRICOLA; PyscINFO; Zoological Records; FSTA (Food Science and Technology Abstracts); GeoRef; DOAJ; 0.4.  Internet access:
  8. The Cardiac atrial appendage stem cell: a new and promising candidate for myocardial repair / Koninckx R, Daniëls A, Windmolders S, Mees U, Macianskiene R, Mubagwa K, Steels P, Jamaer L, Dubois J, Robic B, Hendrikx M, Rummens JL, Hensen K // Cardiovascular research. Oxford: Oxford Journals. ISSN 0008-6363. 2013, vol. 97, no. 3, p. 413-423. Science Citation Index Expanded (Web of Science); MEDLINE; 0.154.  Internet access: