Spausdinti

Laboratory of Cell Culture

 

Laboratory of Cell Culture

Vytenis Arvydas Skeberdis, 

Head of laboratory, Head researcher,  tel.: 302880,

e-mail: arvydas.skeberdis@lsmuni.lt

Personnel:

 Ieva Antanavičiūtė dr.,Senior researcher

 Velasco Sergio Bordel dr., Senior researcher

 Renata Sveikatienė, Junior researcher

Vaidas Jotautis, Junior researcher

 Vytautas Raškevičius, Junior researcher

 Gintarė Milašiūtė,  Laboratory assistant

 

THE MAIN RESEARCH ACTIVITIES OF THE LABORATORY

  • Intracellular and intercellular signaling in cardiac myocytes and cell cultures
  • Stem cell therapy for heart repair

Stem cell therapy is a new rapidly developing research field aiming to regenerate the impaired myocardium and providing the novel strategy for the treatment of cardiovascular disease. We investigate stem cell myogenic characteristics, their viability and ability to differentiate into the cardiac myocytes and integrate into the cardiac tissue. Genetic modifications are being performed to improve viability and intercellular communication in the tissue.

Gap junction channels and membranous tunneling nanotubes provide a direct pathway for electrical, metabolic and genetic signaling between cells. We investigate the formation, permeability and gating properties of gap junction channels and tunneling nanotubes. In particular, we are interested in mechanisms of regulation of gap junction conductance by intracellular pH, protein phosphorylation and energetic state of the cells. Experiments involving structural and functional modeling as well as site-directed mutagenesis are addressed to identify pH sensing domains of the connexins.

We study cellular and molecular mechanisms of b-adrenergic regulation of L-type calcium current and force of contraction of frog, rat and human heart. In cooperation with Laboratory of Cellular and Molecular Cardiology (dr. Rodolphe Fischmeister) of Faculty of Pharmacy of 11th University of Paris we investigate a role of b3-adrenergic receptors and their polymorphism in regulation of L-type calcium current and contraction force of human heart.

We collaborate with Aarhus University (Denmark) investigating the molecular and genetic mechanisms of Short QT syndrome that may cause syncope, ventricular fibrillation or sudden cardiac death.

Technical facilities of the laboratory enable us to perform collaborative research projects with other laboratories working in different scientific fields. In cooperation with Department of Cell and Developmental Biology (University of Colorado, USA) and Proteomics Center (Institute of Biochemistry, Vilnius University) we study the mechanisms regulating midbody inheritance and its role in cancer cell proliferation and invasiveness.

Together with colleagues in the Center of Environmental Research of Vytautas Magnus University and Institute of Experimental and Clinical Pharmacology (Kiel University, Germany) we perform chromatographic, fluorescence microscopy and elecrophysiology studies of stem and cancer cell viability and other properties under stress conditions.

Our partners in the field of regenerative medicine are researchers from Centre for Innovative Medicine (Vilnius) and Institute of Materials Science (Kaunas University of Technology).

Laboratory is hosting the seminars of Intracellular Signaling, where eminent Lithuanian and foreign scientists present their achievements.

Projects:

  1. National Research Programme “Healthy ageing” project “Age-dependent lipid metabolism and cancer cell viability” supported by Lithuanian Research Council grant (SEN-04/2016); 2016-2018; 140.000 Eur.; Principal investigator - Dr. Sergio Bordel Velasco.
  2. Open Partnership project “The mechanisms regulating midbody inheritance and its role in cancer cell proliferation and invasivenes” supported by Lithuanian Research Council grant (APP-7/2016); 2016-2018; 100.000 Eur.; Principal investigator - Prof. V.A.Skeberdis; partner – Prof. R.Prekeris (University of Colorado, USA).
  3. Research Development project “Engineering of biological cardiac pacemaker” in collaboration with Kaunas University of Technology and Centre of Inovative Medicine (Vilnius); supported by Lithuanian Ministry of Education and Science grant (No. VP1-3.1- ŠMM-10-V-02-029); 2014-2015; 1 500 000 Lt.; Co-investigator – Prof. V.A.Skeberdis.
  4. Research project “Development of a quantitative model of tumor progression for clinical applications as a tool to enable optimization of tumor treatment” supported by OlleEnkvistStiftelsen (Sweden ) grant (#21210045); 2014-2016; 220.000 Lt.; Principal investigator – Dr.Sergio Bordel Velasco.
  5. National Research Programme “Chronic Non-Infectious Diseases” project “Formation and properties of intercellular tunneling nanotubes” supported by Lithuanian Research Council; 2012-2014, 690.000 Lt.; Principal investigator - Prof. V.A.Skeberdis.
  6. National Complex Programme “Molecular mechanisms of the ischemic heart disease” supported by European Social Fund (Project Code Number VP1-3.1-ŠMM-0.8-K-01-022), 2012-2014, 220.000 Lt.; Co-investigator - Prof. V.A.Skeberdis.

Main publications (2018-2013): 

2018 -2017 

  1. Femtosecond laser micro-machined polyimide films for cell scaffold applications / Ieva Antanavičiūtė, Linas Šimatonis, Orestas Ulčinas, Aušra Gadeikytė, Brigita Abakevičienė, Sigitas Tamulevičius, Valeryia Mikalayeva, Vytenis Arvydas Skeberdis, Edgaras Stankevičius, Tomas Tamulevičius // Journal of tissue engineering and regenerative medicine. Chichester, West Sussex, UK: John Wiley & Sons. ISSN 1932-6254. 2018, vol. 12, no. 2, p. e760-e773. Science Citation Index Expanded (Web of Science); MEDLINE; 0.52.  Internet access:
  2. Molecular basis for potentiation of Cx36 gap junction channel conductance by n-alcohols and general anesthetics / Vytautas Raškevičius, Vaidas Jotautis, Lina Rimkutė, Alina Marandykina, Mintautė Kazokaitė, Visvaldas Kairys, Vytenis Arvydas Skeberdis // Bioscience reports. London: Portland Press on behalf of the Biochemical Society. ISSN 0144-8463. 2018, vol. 38, no. 1, p. 1-12. Science Citation Index Expanded (Web of Science); MEDLINE; DOAJ; 0.808.  Internet access:
  3. Genome scale metabolic models as tools for drug design and personalized medicine / Vytautas Raškevičius, Valeryia Mikalayeva, Ieva Antanavičiūtė, Ieva Ceslevičienė, Vytenis Arvydas Skeberdis, Visvaldas Kairys, Sergio Bordel // PLoS One. San Francisco: Public Library of Science. (Research article). ISSN 1932-6203. 2018, vol. 13, no. 1, p. 1-14. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; AGRICOLA; 1.237.  Internet access:
  4. Scaffold design for artificial tissue with bone marrow stem cells / Aurelija Noreikaitė, Ieva Antanavičiūtė, Valeryia Mikalayeva, Adas Darinskas, Tomas Tamulevičius, Erika Adomavičiūtė, Linas Šimatonis, Dalia Akramienė, Edgaras Stankevičius // Medicina. Wrocław: Elsevier. (Original research article). ISSN 1010-660X. 2017, vol. 53, no. 3, p. 203-210. Science Citation Index Expanded (Web of Science); MEDLINE; IndexCopernicus; Scopus; 0.444.  Internet access:
  5. Tunneling nanotubes and gap junctions-their role in long-range intercellular communication during development, health, and disease conditions / Jennifer Ariazi, Andrew Benowitz, Vern De Biasi, Monique L. Den Boer, Stephanie Cherqui, Haifeng Cui, Nathalie Douillet, Eliseo A. Eugenin, David Favre, Spencer Goodman, Karine Gousset, Dorit Hanein, David I. Israel, Shunsuke Kimura, Robert B. Kirkpatrick, Nastaran Kuhn, Claire Jeong, Emil Lou, Robbie Mailliard, Stephen Maio, George Okafo, Matthias Osswald, Jennifer Pasquier, Roel Polak, Gabriele Pradel, Bob de Rooij, Peter Schaeffer, Vytenis A. Skeberdis, Ian F. Smith, Ahmad Tanveer, Niels Volkmann, Zhenhua Wu, Chiara Zurzolo // Frontiers in molecular neuroscience. Lausanne, Switzerland: Frontiers Research Foundation. (Review article). ISSN 1662-5099. 2017, vol. 10, p. 1-12. Prieiga per internetą: <https://www.ncbi.nlm.nih.gov/pubmed/29089870>. [Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; 0.145. Internet access:
  6. Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism / Ieva Antanavičiūtė, Valeryia Mikalayeva, Ieva Ceslevičienė, Gintarė Milašiūtė, Vytenis Arvydas Skeberdis, Sergio Bordel // Scientific reports. London: Nature Publishing Group. ISSN 2045-2322. 2017, vol. 7, no. 1, p. 1-12. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; 1.414.   Internet access:
  7. Loss-of-activity-mutation in the cardiac chloride-bicarbonate exchanger AE3 causes short QT syndrome / Kasper Thorsen, Vibeke S. Dam, Kasper Kjaer-Sorensen, Lisbeth N. Pedersen, V. Arvydas Skeberdis, Jonas Jurevičius, Rimantas Treinys, Ida M.B.S. Petersen, Morten S. Nielsen, Claus Oxvig, J. Preben Morth, Vladimir V. Matchkov, Christian Aalkjær, Henning Bundgaard, Henrik K. Jensen // Nature communications. London: Nature Publishing Group. ISSN 2041-1723. 2017, vol. 8, no. 1, p. 1-10. Science Citation Index Expanded (Web of Science); MEDLINE; DOAJ; 0.529.  Internet access:
  8. FYCO1 regulates accumulation of post-mitotic midbodies by mediating LC3-dependent midbody degradation / Lai Kuan Dionne, Eric Peterman, John Schiel, Paulius Gibieža, Vytenis Arvydas Skeberdis, Antonio Jimeno, Xiao-Jing Wang, Rytis Prekeris // Journal of cell science. Cambridge: Company of Biologists. (Research article). ISSN 0021-9533. 2017, vol. 130, no. 23, p. 4051-4062. [Science Citation Index Expanded (Web of Science); MEDLINE; CAB abstracts; Scopus; 0.433. Internet access :
  9. Symmetries and nonequilibrium thermodynamics / Bordel S // Physical Review. E (PRE). Ridge, NY: American Physical Society. ISSN 2470-0045. 2017, vol. 95, p. 1-10. Science Citation Index Expanded (Web of Science); MEDLINE; 1.414.  Internet access:

2016 -2015

  1. Differences in the control of basal L-type Ca2+ current by the cyclic AMP signaling cascade in frog, rat, and human cardiac myocytes / Rimantas Treinys, Andrius Bogdelis, Lina Rimkutė, Jonas Jurevičius, Vytenis Arvydas Skeberdis // Journal of physiological sciences : JPS. Tokyo: Springer. (Original paper). ISSN 1880-6546. 2016, vol. 66, no. 4, p. 327-336. Science Citation Index Expanded (Web of Science); MEDLINE; Chemical Abstracts Service (CAS); CAB International; Scopus; 0.8. Internet access:
  2. The Role of neural connexins in HeLa cell mobility and intercellular communication through tunneling tubes / Lina Rimkutė, Vaidas Jotautis, Alina Marandykina, Renata Sveikatienė, Ieva Antanavičiūtė, Vytenis Arvydas Skeberdis // BMC cell biology. London: Biomed Central Ltd. (Research article). ISSN 1471-2121. 2016, vol. 17, no. 1, p. 1-12. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; DOAJ; 0.833. Internet access:
  3. Relevance of HCN2-expressing human mesenchymal stem cells for the generation of biological pacemakers / Ieva Bruzauskaite, Daiva Bironaite, Edvardas Bagdonas, Vytenis Arvydas Skeberdis, Jaroslav Denkovskij, Tomas Tamulevicius, Valentinas Uvarovas, Eiva Bernotiene // Stem cell research & therapy. London: BioMed Central. (Research). ISSN 1757-6512. 2016, vol. 7, no. 1, p. 1-15. Science Citation Index Expanded (Web of Science); MEDLINE; CAS; DOAJ; Scopus; 0.25. Internet access:
  4. Reverberation of excitation in neuronal networks interconnected through voltage-gated gap junction channels/ Kestutis Maciunas, Mindaugas Snipas, Nerijus Paulauskas, Feliksas F. Bukauskas // Journal of general physiology. New York: Rockefeller University Press. (Research article). ISSN 0022-1295. 2016, vol. 147, iss. 3, p. 273-288. Science Citation Index Expanded (Web of Science); MEDLINE; BIOSIS Previews; 1.5. Internet access:
  5. Stochastic model of gap junctions exhibiting rectification and multiple closed states of slow gates / Mindaugas Snipas, Tadas Kraujalis, Nerijus Paulauskas, Kestutis Maciunas, Feliksas F. Bukauskas // Biophysical journal. Cambridge, MA: Cell Press. ISSN 0006-3495. 2016, vol. 110, no. 6, p. 1322-1333. Science Citation Index Expanded (Web of Science); MEDLINE; 2. Internet access:
  6. Exogenous connexin43-expressing autologous skeletal myoblasts ameliorate mechanical function and electrical activity of the rabbit heart after experimental infarction / Antanavičiūtė I, Ereminienė E, Vysockas V, Račkauskas M, Skipskis V, Rysevaitė K, Treinys R, Benetis R, Jurevičius J, Skeberdis VA // International journal of experimental pathology. Oxford, OX: Blackwell Scientific Publications. (Original article). ISSN 0959-9673. 2015, vol. 96, no. 1, p. 42-53.Science Citation Index Expanded (Web of Science); MEDLINE; Index Veterinarius; CAB Abstracts® (CABI); 1.342. Internet access:
  7. Application of stochastic automata networks for creation of continuous time markov chain models of voltage gating of gap junction channels / Mindaugas Snipas, Henrikas Pranevicius, Mindaugas Pranevicius, Osvaldas Pranevicius, Nerijus Paulauskas, Feliksas F. Bukauskas // BioMed research international. New York: Hindawi Publishing Corporation. (Research article). ISSN 2314-6133. 2015, vol. 2015, p. 1-13. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; 0.408. Internet access:

2014-2013

  1. Long-distance communication between laryngeal carcinoma cells / Antanavičiūtė I, Rysevaitė K, Liutkevičius V, Marandykina A, Rimkutė L, Sveikatienė R, Uloza V, Skeberdis VA // PLoS One. San Francisco: Public Library of Science. ISSN 1932-6203. 2014, vol. 9, no. 6, p. 1-15. Science Citation Index Expanded (Web of Science); MEDLINE; Scopus; AGRICOLA; PyscINFO; Zoological Records; FSTA (Food Science and Technology Abstracts); GeoRef; DOAJ; 1.25. Internet access:
  2. Continuous time Markov chain models of voltage gating of gap junction channels / Henrikas Pranevicius, Mindaugas Pranevicius, Osvaldas Pranevicius, Mindaugas Snipas, Nerijus Paulauskas, Feliksas Bukauskas // Information technology and control = Informacinės technologijos ir valdymas / Kaunas: KTU. ISSN 1392-124X. 2014, vol. 43, no. 2, p. 133-142. Science Citation Index Expanded (Web of Science); INSPEC; VINITI; Scopus; 0.408. Internet access:
  3. β3-adrenergic regulation of L-Type Ca2+ current and force of contraction in human ventricle / Treinys R, Zablockaitė D, Gendvilienė V, Jurevičius J, Skeberdis VA // Journal of membrane biology. New York: Springer. ISSN 0022-2631. 2014, vol. 247, iss. 4, p. 309-318. Science Citation Index Expanded (Web of Science); MEDLINE; 0.6. Internet access:
  4. Hyperthermia differently affects connexin43 expression and gap junction permeability in skeletal myoblasts and HeLa cells / Antanavičiūtė I, Mildažienė V, Stankevičius E, Herdegen T, Skeberdis VA // Mediators of inflammation. Sylvania: Hindawi Publishing Corporation. (Research article). ISSN 0962-9351. 2014, vol. 2014, p. 1-16. Science Citation Index Expanded (Web of Science); MEDLINE; 0.894. Internet access:
  5. Regulation of connexin-36 gap junction channels by n-alkanols and arachidonic acid / Marandykina A, Palacios-Prado N, Rimkute L, Skeberdis VA, Bukauskas FF // Journal of physiology. Oxford: Blackwell: Cambridge Univ. Press. ISSN 0022-3751. 2013, vol. 591, part 8, p. 2087-2101. Science Citation Index Expanded (Web of Science); MEDLINE; 0.849. Internet access :
  6. Intracellular magnesium-dependent modulation of gap junction channels formed by neuronal connexin36 / Palacios-Prado N, Hoge G, Marandykina A, Rimkute L, Chapuis S, Paulauskas N, Skeberdis VA, O'Brien J, Pereda AE, Bennett MV, Bukauskas FF // Journal of neuroscience. Washington, DC: Society for Neuroscience. (Cellular/Molecular). ISSN 0270-6474. 2013, vol. 33, iss. 11, p. 4741-4753. Science Citation Index Expanded (Web of Science); MEDLINE; BIOSIS; Brain Information Service; Chemical Abstracts; FISHLIT; Index Medicus; Psychological Abstracts; PsycINFO; PsycLIT; 0.813. Internet access: